FastQCFastQC Report
Mon 6 Jun 2016
ERR1378785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699406
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGACTATCTCGTATGCCGTC16070.22976640177522067Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGACTATCTCGTATGCC14530.20774771734872163Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1650.040.90681543
ATGCCGT1850.036.4844644
TGCCGTC1900.035.52433845
CGCCCGG702.7021988E-522.49874913
TCTCGTA3400.020.51356538
ACGCCCG550.003936288420.4534112
CTCGTAT3300.020.45340739
AGACCGT3300.020.45340725
TCGTATG3300.020.45340740
CGTATGC3350.020.14813241
GACCGTT3400.019.85183726
TATCTCG3450.019.5641336
ATCTCGT3500.019.28464337
CGTTGAC3500.019.28464329
GTATGCC3850.019.28464142
GAGACCG3550.019.01302724
ACCGTTG3550.019.01302727
CCGTTGA3550.019.01302728
GACTATC3550.019.01302733
GCGGTTC5500.018.8238645
Produced by FastQC (version 0.11.5)