FastQCFastQC Report
Mon 6 Jun 2016
ERR1378782.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378782.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839107
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAACTATCTCGTATGCCGTC13390.15957440469451453Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAACTATCTCGTATGCC12860.15325816612184143Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1650.036.81598743
TGCCGTC1700.035.73316645
ATGCCGT1750.034.71221544
GATCGGA957.421477E-1026.05107926
AGATCGG1001.3442332E-924.74852425
ATGCCGA3950.022.78345319
ATCGGAA1204.5110937E-1022.4986627
AATGCCG4150.021.68545318
TGCCGAG4100.021.40116520
CCGTCAA3050.021.39216828
CGTCAAC2950.021.3546629
AGACCGT3000.020.99874725
CCGAGAC3000.020.99874722
CTCGTAT2950.020.59199539
CGTTCTC550.003926166320.4630811
GACCGTC3100.020.32137126
AGCGGTT4550.020.2807674
CGGTTCA4500.020.005966
TCGGAAG1351.9626896E-919.9988128
GAGACCG3150.019.99880824
Produced by FastQC (version 0.11.5)