FastQCFastQC Report
Mon 6 Jun 2016
ERR1378781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891100
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTTATATCTCGTATGCCGTC9430.10582426214790708Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1950.036.92243243
ATGCCGT1950.036.92243244
TGCCGTC2000.035.9993745
CGCGTTA2150.033.48778529
GCGTTAT2250.031.9994430
CCGCGTT2250.031.9994428
CGTATGC2300.031.30380241
CTCGTAT2300.030.32555839
TCTCGTA2400.029.06199338
ACCGCGT2500.028.79949627
TCGTATG2500.028.79949640
CGTTATA2600.028.55719431
TATCTCG2450.028.46889336
AGACCGC2750.026.99952925
ATCTCGT2600.026.82645437
GAGACCG2750.026.18136224
ATGCCGA3800.026.05217719
GACCGCG2850.025.26271826
AATGCCG3950.025.06285518
TGCCGAG4100.024.14592220
Produced by FastQC (version 0.11.5)