FastQCFastQC Report
Mon 6 Jun 2016
ERR1378780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853762
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTTATATCTCGTATGCCGTC9430.1104523274636257Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTTATATCTCGTATGCC9280.10869539754638881Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1350.039.99703243
ATGCCGT1550.036.28763244
TGCCGTC1800.029.99777445
CTCGTAT2250.023.9982239
ACCGGCT2350.022.97701827
TCGTATG2400.022.49833140
CGGCTTA2400.022.49833129
CGTATGC2450.022.03918341
TCTCGTA2450.022.03918338
CCGGCTT2550.021.174928
TATCTCG2550.021.174936
GACCGGC2600.020.76768926
GAGACCG2600.020.76768924
ATCTCGT2700.019.99851637
CCGGCTA807.564402E-519.68603916
CGGCTAA807.564402E-519.68603917
AGACCGG2750.019.63490725
TGCCGAG3950.019.36565220
AATGCCG4100.019.20589318
ATGCCGA4050.018.88748719
Produced by FastQC (version 0.11.5)