FastQCFastQC Report
Mon 6 Jun 2016
ERR1378779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001781
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTACATCTCGTATGCC14850.14823599169878449Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTACATCTCGTATGCCGTC12300.12278132645757904Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1600.037.9655543
TGCCGTC2350.025.84888645
ATGCCGT2450.025.71211844
TCGTATG3350.022.83389543
CTCGTAT3300.022.49810442
CGACTTA3400.022.49810232
CGTATGC3550.022.18122944
ACCGACT3450.022.17204330
CCGACTT3650.020.95713631
TCTCGTA3700.020.06587641
GACCGAC3950.018.79588329
ATCGGAA1951.8189894E-1218.45998227
AGACCGA4100.018.10822928
ATCTCGT4250.017.99848440
CGGACGA750.001110715817.99848423
ATGCCGA5900.017.54089519
GTATGCC4700.017.23259245
CGGAAGA4600.017.1292361
GACTTAC4500.016.99856833
TCGGAAG2251.8189894E-1216.99856828
Produced by FastQC (version 0.11.5)