FastQCFastQC Report
Mon 6 Jun 2016
ERR1378777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933313
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGATATCTCGTATGCCGTC14100.15107471984211085Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGATATCTCGTATGCC13710.1468960573783929Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1550.037.7403643
TGCCGTC1550.037.7403645
GCGATTA250.00210733735.998532
ATGCCGT1750.033.4271844
CGTATGC2950.022.88040444
AGACCGC3000.022.49906228
CTCGTAT3200.021.0928742
TATCTCG3200.021.0928739
GACCGCT3100.021.04750829
GAGACCG3250.020.76836627
TCTCGTA3250.020.76836641
TCGTATG3250.020.76836643
ACCGCTT3150.020.71342330
CCGCTTG3250.020.07608631
CGGAAGA3500.019.9330831
ATCTCGT3500.019.28491240
GTATGCC3500.019.28491245
AGATCGG1454.771209E-918.61991517
ACGCCCG750.001110321317.9992512
CGCTCTT750.001110321317.9992520
Produced by FastQC (version 0.11.5)