FastQCFastQC Report
Mon 6 Jun 2016
ERR1378774.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378774.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878812
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCC12240.13927893565404204Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCCGTC11160.1269896178022148Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1600.036.5601943
ATGCCGT1650.035.452344
TGCCGTC1650.035.452345
ACCGTTA2750.021.27138327
CCGGCTA754.6032925E-520.99867216
GACCGTT2800.020.89153526
GAGACCG2800.020.89153524
CGTATGC2950.020.59191741
AGACCGT2850.020.52501925
CGGAAGA3250.020.0870821
CTCGTAT2950.019.82925439
CGTTACA2950.019.82925429
TCGTATG2950.019.82925440
TCTCGTA3000.019.49876638
ACGCCCG706.984651E-419.28449412
CCGTTAC3100.018.86977428
CGCCCGG1102.2561126E-618.40792713
GCGGTTC4550.018.3028355
ATCTCGT3200.018.28009437
GTATGCC3200.018.28009442
Produced by FastQC (version 0.11.5)