FastQCFastQC Report
Mon 6 Jun 2016
ERR1378772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254601
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1450.035.68743
TGCCGTC1550.033.38461345
ATGCCGT1850.027.97089244
CGACAAA450.001227149424.9981420
CGCAATC500.002265179522.49922415
TCTCGTA2350.022.02051538
GACCGTA2500.021.59925526
TCGTATG2450.021.12172140
CGCGCCC754.6057034E-520.99843811
CCGTAAG2600.020.7693428
CGTATGC2500.020.6984641
CATTACG550.003938431420.45302411
AGACCGT2650.020.37665625
CTCGTAT2550.020.29341539
ACCGTAA2700.020.00010727
GCGCCCG908.257544E-619.99851212
CGTAAGT2750.019.63646929
TGCGCCT600.00650313418.7538364
GAGACCG2900.018.62004724
ATCTCGT2800.018.48150437
Produced by FastQC (version 0.11.5)