FastQCFastQC Report
Mon 6 Jun 2016
ERR1378770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998785
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAATAATCTCGTATGCC12270.12284926185315158Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAATAATCTCGTATGCCGTC11950.11964536912348503Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1300.034.61388443
TGCCGTC1450.031.03313645
ATGCCGT1550.029.03099844
CTCGTAT2450.022.03986242
TCTCGTA2600.021.63367741
ACCGGTA2550.021.17555230
TCGTATG2600.020.7683343
CGGAAGA2500.020.7063581
CCGACCA550.00393717120.4536636
CGTATGC2650.020.37647444
GACCGGT2750.019.63551129
GATCGGA1151.5955447E-719.5643726
ATCTCGT2800.019.28487640
CCGGTAA2800.019.28487631
CGGTAAT2950.019.06696932
AGACCGG2850.018.94654728
CAGGTCG600.006500259518.754823
CCGAGAC2900.018.6198825
CCACGCC1751.382432E-1017.9992210
GAGACCG3000.017.99921827
Produced by FastQC (version 0.11.5)