FastQCFastQC Report
Mon 6 Jun 2016
ERR1378766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430297
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC552.741217E-936.81760844
CTCGTAT552.741217E-936.81760842
TCTCGTA552.741217E-936.81760841
TCGTATG552.741217E-936.81760843
ACCGATT606.4137566E-933.74947730
CCGATTG651.39953045E-831.15336231
GACCGAT651.39953045E-831.15336229
GATCGTG300.0051441129.9995374
CGATTGA702.8778231E-828.92812232
GAGACCG755.62286E-826.99958227
AGAGCGG755.62286E-826.9995825
CGGAAGA856.4428605E-926.4763321
CGGTTCA856.4592314E-926.4701799
GCGGTTC801.0507574E-725.3121078
AGCGGTT801.0507574E-725.3121077
GAGCGGT801.0507574E-725.3121076
AATCTCG801.0507574E-725.31210739
ATGCCGA801.0507574E-725.31210722
TGCCGAG801.0507574E-725.31210723
ATCTCGT851.888202E-723.8231640
Produced by FastQC (version 0.11.5)