FastQCFastQC Report
Mon 6 Jun 2016
ERR1378763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447025
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGC207.0303766E-444.9977630
CGTATGC301.1391394E-437.4981444
CTCGTAT301.1391394E-437.4981442
TCGTATG301.1391394E-437.4981443
TCTCGTA352.8184595E-432.14126241
TGCTCGC300.005145183829.9985137
ATCTCGT406.161993E-428.12360240
GTCGCCC406.161993E-428.1236029
CGAGTAG551.364763E-424.5497265
AGCGGTT551.3668377E-424.5442357
CCGAGTA602.4613363E-422.5039144
AGAGCGG602.4613363E-422.5039145
GCGGTTC602.4650674E-422.4988828
CGGTTCA602.4650674E-422.4988829
CGGGATT802.9692928E-622.4988814
TCGTTGG855.013295E-621.1801574
CGTTGGA855.013295E-621.1801575
AAGAGCG654.227679E-420.7728484
GAGCGGT654.230873E-420.7705216
ACGGGAT550.003933965720.4535313
Produced by FastQC (version 0.11.5)