FastQCFastQC Report
Mon 6 Jun 2016
ERR1378762.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378762.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715732
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG502.1827873E-1144.9987443
CGAGTAA501.0804797E-940.49886732
CGTATGC653.6743586E-1034.61441444
ACCGAGT651.4024408E-831.15297330
TATCTCG651.4024408E-831.15297339
TCTCGTA651.4024408E-831.15297341
CAAACGT300.005144921630.0012555
GAGCGGT803.3887773E-928.1261796
ATCTCGT755.6345016E-826.99924740
CTCGTAT755.6345016E-826.99924742
GAGACCG755.6345016E-826.99924727
AGCGGTT901.189801E-824.9993027
AGAGCGG851.8908031E-723.824535
AGACCGA851.8920764E-723.82286528
GCGGTTC1003.6405254E-822.499378
CGAGACC1003.6405254E-822.4993726
GACCGAG1009.047544E-720.24943429
CCGAGAC1009.047544E-720.24943425
AAGAGCG1051.4434318E-619.2865244
AGCGCTC706.981258E-419.2851757
Produced by FastQC (version 0.11.5)