FastQCFastQC Report
Mon 6 Jun 2016
ERR1378761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720387
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA751.8189894E-1235.99866529
TCTCGTA751.8189894E-1235.99866541
CCGAACA751.8189894E-1235.99866531
ATCTCGT803.6379788E-1233.7487540
CTCGTAT803.6379788E-1233.7487542
ACCGAAC803.6379788E-1233.7487530
CGTATGC855.456968E-1231.7635344
TATCTCG855.456968E-1231.7635339
TCGTATG855.456968E-1231.7635343
GCGGTTC901.2732926E-1129.9988888
GAGACCG952.3646862E-1128.42000227
AGAGCGG1004.5474735E-1127.0046235
AGACCGA1004.5474735E-1126.99928
AAGAGCG1105.456968E-1226.595464
AATGCCG1058.367351E-1125.71333321
CCGAGAC1159.094947E-1225.43384225
GAGCGGT1101.5097612E-1024.5496566
CGAACAA1101.5097612E-1024.54454432
CGGTTCA1201.6370905E-1124.37419
AGCGGTT1152.6557245E-1023.4773927
Produced by FastQC (version 0.11.5)