FastQCFastQC Report
Mon 6 Jun 2016
ERR1378759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772404
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT403.4567347E-739.3743230
GACCGGA458.696188E-734.99939729
CGGATGT501.9807721E-631.49945632
TCGTATG501.9817744E-631.49741743
CGGGGAC300.005138518330.0091975
CGTATGC554.165271E-628.63401444
ATCTCGT608.188097E-626.24954640
CCGGATG608.188097E-626.24954631
CTCGTAT608.192215E-626.24784942
GAGCGGT651.5200621E-524.2366266
AGCGGTT651.5231144E-524.230357
CGGAAGA702.6964697E-522.5054381
GCGGTTC702.7018708E-522.499618
TCTCGTA702.7032225E-522.49815441
AGAGCGG807.541112E-519.6935355
AGACCGG807.559902E-519.6871628
CGCCACT1102.2557997E-618.4075819
CCGAGAC750.001109903617.9996925
CGGTTCA901.8658469E-417.4985669
AATGCCG850.002564438915.88207821
Produced by FastQC (version 0.11.5)