FastQCFastQC Report
Mon 6 Jun 2016
ERR1378755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526435
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCGA250.00210522236.0029641
CGGACAT453.4875895E-529.9996232
GCGGTTC453.4875895E-529.999628
TCTCGTA406.162125E-428.12464341
TCGTATG406.162125E-428.12464343
GACCGGA507.1517694E-526.99965929
AGCGGTT507.1517694E-526.9996597
CGTATGC450.001225762224.99968344
AAGAGCG551.3660635E-424.5474744
AGACCGG551.3669446E-424.54514328
GAGCGGT602.463672E-422.5018526
AGAGCGG602.463672E-422.5018525
CGGAAGA754.5936344E-521.0017281
ACCGGAC654.2343885E-420.76896930
CGGTTCA654.2343885E-420.7689699
GAGACCG654.2343885E-420.76896927
ACCACGC1051.4427042E-619.285479
CTCGTAT600.006506394618.74976242
AATGCCG851.202984E-418.52917721
CGGGGTT1102.2494878E-618.4106061
Produced by FastQC (version 0.11.5)