FastQCFastQC Report
Mon 6 Jun 2016
ERR1378753.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378753.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883541
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG754.7293724E-1132.99842543
CGTATGC751.7025741E-929.99856844
GACCGAG751.7025741E-929.99856829
AGACCGA856.4810592E-926.46932228
ACCGAGC952.1151209E-823.6830830
GAGACCG952.1151209E-823.6830827
GCGGTTC1003.639434E-822.501478
CTCGTAT1003.6437996E-822.49892442
TCTCGTA1056.106893E-821.42754741
CGGTTCA1109.982614E-820.453579
CGTTGGA1603.8198777E-1119.6910175
AATGCCG1151.5951082E-719.56428321
AGCGGTT1302.7679562E-819.0397077
CCGAGAC1302.7715942E-819.03755425
AGAGCGG1202.490342E-718.7533515
ACGTATA600.00651112818.74910416
GAGCGGT1253.8235703E-718.0032166
ATCTCGT1253.8326652E-717.9991440
CGGCTAA901.8657424E-417.49916517
AAGAGCG1358.5608735E-716.6696434
Produced by FastQC (version 0.11.5)