FastQCFastQC Report
Mon 6 Jun 2016
ERR1378752.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378752.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603484
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC351.2108649E-744.9989244
CGGATCG458.692441E-734.9991632
CTCGTAT551.1264456E-732.7264942
GCGGTTC501.9799263E-631.4992498
GGATCGA501.9799263E-631.49924933
TCTCGTA501.9799263E-631.49924941
CGAATCT501.9799263E-631.49924937
TCGTATG602.4162728E-729.9992843
ACCGGAT554.1614057E-628.63567730
GATCGAA554.1614057E-628.63567734
CCGGATC554.1614057E-628.63567731
CGGTTCA608.1846465E-626.249379
GACCGGA608.1846465E-626.2493729
AGACCGG608.1846465E-626.2493728
TCGAATC608.1846465E-626.2493736
AAGAGCG651.5214986E-524.2321954
ATCTCGT651.5224756E-524.23018840
GAGCGGT702.6990163E-522.5013266
AGCGGTT702.7007449E-522.499467
CGAGGTT550.003929288620.459143
Produced by FastQC (version 0.11.5)