FastQCFastQC Report
Mon 6 Jun 2016
ERR1378751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593442
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT850.042.35064342
TCTCGTA850.042.35064341
CGGTACG800.042.1852132
CCGGTAC850.039.70372831
GTACGAA850.039.70372834
TCGTATG1000.038.2479243
TACGAAT1000.035.99804335
GGTACGA950.035.52438733
ACCGGTA950.035.52438730
ACGAATC950.035.52438736
AGAGCGG1100.034.7825625
CGTATGC1050.034.2838544
GACCGGT1000.033.74816529
CGAATCT1050.032.1411137
CGGAAGA1150.031.3132021
GCGGTTC1150.031.3079248
AGACCGG1100.030.68015128
CATGCGC300.00513816530.0084861
GAGCGGT1200.030.0059576
ATCCGGG300.005146641729.99837323
Produced by FastQC (version 0.11.5)