FastQCFastQC Report
Mon 6 Jun 2016
ERR1378750.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378750.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912458
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA602.417546E-730.00072941
TATCTCG602.4187102E-729.99908639
CGAGACG602.4187102E-729.99908632
AGCGGTT702.8856448E-828.9276927
GAGACCG702.8856448E-828.92769227
CTCGTAT709.302039E-725.71491242
CGTATCT709.3064773E-725.71350537
GACCGAG709.3064773E-725.71350529
ACGTATC709.3064773E-725.71350536
CGGAAGA751.6934137E-624.0058461
GAGCGGT751.6958384E-624.00196
GACGTAT751.6974573E-623.9992735
CGGTTCA751.6974573E-623.999279
ATCTCGT802.9730109E-622.50054640
GCGGTTC802.974426E-622.4993158
ACCGAGA802.974426E-622.49931530
AATGCCG702.7028387E-522.49931521
GAGACGT802.974426E-622.49931533
TCCGTAG500.00226465622.49931517
ATGCCGA702.7028387E-522.49931522
Produced by FastQC (version 0.11.5)