FastQCFastQC Report
Mon 6 Jun 2016
ERR1378744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630035
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG504.8748916E-835.99841343
CTCGTAT501.980421E-631.49861142
TATCTCG501.980421E-631.49861139
CGTATGC602.4169822E-729.99867844
GAGCGGT554.15473E-628.6419226
TCTCGTA554.162439E-628.63510141
GACCGCA654.865997E-727.69328729
CGCAGAG608.186675E-626.24884232
AGAGCGG651.5200443E-524.2354725
GAGACCG651.5219148E-524.23162727
ATCTCGT702.7014088E-522.49900840
CCGCAGA702.7014088E-522.49900831
ACCGCAG754.5971443E-521.00074230
AGACCGC754.5971443E-521.00074228
GCGGTTC754.599957E-520.9990738
GTATGCC754.599957E-520.99907345
AGCGGTT807.558636E-519.6866327
AATGCCG851.20375145E-418.52859521
AAGAGCG952.8126515E-416.5821654
CGGTTCA952.8177656E-416.5782179
Produced by FastQC (version 0.11.5)