FastQCFastQC Report
Mon 6 Jun 2016
ERR1378743.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378743.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734963
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC700.041.78495844
GACCGTG700.041.78495829
GCGGTTC800.036.5618368
GAGACCG800.036.56183627
CCGTGCA850.034.4111431
AGACCGT850.034.4111428
AGCGGTT900.032.4994137
TCTCGTA950.030.78891641
CGGTTCA1050.029.9994569
TCGTATG1001.8189894E-1229.2494743
AGAGCGG1105.456968E-1226.5922365
ATCTCGT1105.456968E-1226.59042540
CTCGTAT1105.456968E-1226.59042542
ACCGTGC1105.456968E-1226.59042530
GAGCGGT1159.094947E-1225.436056
GTATGCC1159.094947E-1225.43432245
ATGCGCC450.001224805125.004652
AAGAGCG1201.6370905E-1124.3762174
CGTATAA802.9728017E-622.49959210
CCGAGAC1358.367351E-1121.66627525
Produced by FastQC (version 0.11.5)