FastQCFastQC Report
Mon 6 Jun 2016
ERR1378740.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378740.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451625
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT453.8380676E-1045.0036546
AAGAGCG501.0768417E-940.503294
CGGAAGA552.733941E-936.8293271
AGCGGTT552.743036E-936.8170937
CTCGTAT504.869071E-835.9989442
CGGTTCA653.6561687E-1034.614369
CGTATGC551.1254269E-732.72630744
GACCGAG551.1254269E-732.72630729
ACCGAGT551.1254269E-732.72630730
TCTCGTA551.1254269E-732.72630741
CCGAGAC551.1254269E-732.72630725
AATGCCG602.4141082E-729.99911321
TCGTATG602.4141082E-729.99911343
AGAGCGG702.8758222E-828.930925
GAGACCG654.863614E-727.6914927
AGACCGA709.2889786E-725.71352628
GCGGTTC801.0511394E-725.3117528
CGTTGCT450.001225612724.99926229
ATCTCGT802.9688854E-622.49933640
CGAGTGC802.9688854E-622.49933632
Produced by FastQC (version 0.11.5)