FastQCFastQC Report
Mon 6 Jun 2016
ERR1378736.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378736.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689958
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC556.002665E-1140.90915744
GACCGCA702.1827873E-1135.35720429
CGCAGTA653.6743586E-1034.6154432
CCGCAGT754.7293724E-1132.9976631
CTCGTAT708.1308826E-1032.1429142
AGACCGC708.1308826E-1032.1429128
TCGTATG708.1308826E-1032.1429143
AGAGCGG751.6989361E-930.0022225
TCTCGTA751.7007551E-930.0000541
ACCGCAG803.3905962E-928.12504630
GCGGTTC803.3924152E-928.1230078
AATGCCG803.3924152E-928.12300721
GAGACCG856.4719643E-926.4706327
CGGTTCA856.4774213E-926.4687129
ATCTCGT957.403287E-1026.05267140
GAGCGGT901.1883458E-825.0018546
CCGAGAC901.1892553E-825.0000425
AGCGGTT952.1138476E-823.682537
ATGCCGA952.1138476E-823.6825322
CGGAAGA1056.0754246E-821.4363731
Produced by FastQC (version 0.11.5)