FastQCFastQC Report
Mon 6 Jun 2016
ERR1378732.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378732.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864195
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC651.4029865E-831.15308444
GCGGTTC702.883462E-828.9295378
CTCGTAT755.637048E-826.9993442
CGCATGA755.637048E-826.9993432
GAGCGGT801.0522308E-725.314816
AGAGCGG801.0522308E-725.314815
TCGTATG801.05341314E-725.31188243
ATCTCGT901.1905286E-824.9993940
AAGAGCG851.8908577E-723.8257034
AGCGGTT851.8919127E-723.8243267
ACCGCAT851.8929677E-723.82294730
GACCGCA851.8929677E-723.82294729
TCTCGTA851.8929677E-723.82294741
AGACCGC851.8929677E-723.82294728
CGGAAGA1003.635978E-822.5033551
CGGTTCA903.2857497E-722.499459
CCGCATG903.2857497E-722.4994531
GAGACCG1051.4450579E-619.28524427
GCGCCCG600.00651013918.7495412
ATGCCGA1102.2551885E-618.40864222
Produced by FastQC (version 0.11.5)