FastQCFastQC Report
Mon 6 Jun 2016
ERR1378731.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378731.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780442
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG302.1651758E-644.9982731
GACCGAT453.490338E-529.99884829
CGATCGA507.157378E-526.9989632
ATCTCGT551.36801E-424.5445140
AGACCGA551.36801E-424.5445128
CGTATGC500.002264469622.49913444
CTCGTAT500.002264469622.49913442
GATCGAG602.467167E-422.49913433
AGCGGTT602.467167E-422.4991347
TCTCGTA602.467167E-422.49913441
TCGAGAT602.467167E-422.49913435
GAGACCG754.601563E-520.99919327
GCGGTTC654.2376498E-420.7684338
ACCGATC654.2376498E-420.76843330
GAGCGGT706.976507E-419.2874456
TCGTATG600.006510113418.74927943
CGTGTTA600.006510113418.74927910
CGGTTCA750.001110075317.999319
CCGAGAC750.001110075317.9993125
CCGCCAC1053.1079584E-517.1443964
Produced by FastQC (version 0.11.5)