FastQCFastQC Report
Mon 6 Jun 2016
ERR1378730.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378730.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789052
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC401.5604306E-533.7484844
GATCGGA406.166042E-428.12373418
ATCTCGT551.3680899E-424.5443540
CTCGTAT551.3680899E-424.5443542
GACCGTG654.2360858E-420.76961129
AGACCGT654.2360858E-420.76961128
ACCGTGA654.2360858E-420.76961130
CATCTCG654.2378978E-420.76829339
TCGTATG654.2378978E-420.76829343
CGTTGGA1751.382432E-1018.001475
GAGACCG750.001109667518.0003327
CCGAGAC750.001109667518.0003325
TCTCGTA750.001110139517.9991941
CGGGATT1406.4716005E-817.67777414
ACGGGAT1305.779639E-717.30691113
TTCGTTG1853.0559022E-1017.0305773
GAGCGGT800.001709268816.8763796
CGTGAGA800.001709993816.87530932
GTATGCC800.001710719116.8742445
CCACCGC1104.63634E-516.36297
Produced by FastQC (version 0.11.5)