FastQCFastQC Report
Mon 6 Jun 2016
ERR1378727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805297
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAG603.6379788E-1241.24846330
GACCGGA659.094947E-1238.0755129
CTCGTAT601.546141E-1037.49860442
CGTATGC552.748493E-936.8168144
TATCTCG803.6379788E-1233.74874539
ATCTCGT855.456968E-1231.76352340
GAGCGGT801.0004442E-1030.942116
AGAGCGG801.0004442E-1030.942115
TCTCGTA801.0004442E-1030.93634841
CGGAAGA903.929017E-1027.5092221
GCGGTTC903.947207E-1027.4989768
AGCGGTT903.947207E-1027.4989767
AGACCGG903.947207E-1027.49897628
TCGTATG755.6368663E-826.99899543
CCGGAGC1004.5474735E-1126.99899331
CGGTTCA957.421477E-1026.051669
CGGAGCA957.421477E-1026.0516632
GGAGCGT450.001226502224.9990728
GAGACCG1001.3442332E-924.74907727
AAGAGCG1104.0417945E-922.5033534
Produced by FastQC (version 0.11.5)