FastQCFastQC Report
Mon 6 Jun 2016
ERR1378725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729072
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC551.1271368E-732.7259844
GCGGTTC551.1271368E-732.725988
GATCGTT300.005147035729.99881212
GACCGTC602.4177643E-729.99881229
CGGTTCA702.8843715E-828.9274239
CGGAAGA654.8505535E-727.704511
CGTCTGT654.8709626E-727.69121232
GAGACCG654.8709626E-727.69121227
AGAGCGG709.28625E-725.7185575
TCGTTGG1400.024.1111474
GAGCGGT751.6937756E-624.0039866
CTCGTAT751.6968115E-623.99904842
CCGTCTG751.6968115E-623.99904831
AGCGGTT751.6968115E-623.9990487
TCTCGTA751.6968115E-623.99904841
AGACCGT751.6968115E-623.99904828
ACCGTCT751.6968115E-623.99904830
ATGCCGA903.2847856E-722.4991122
TGCCGAG802.9733E-622.4991123
CGTTGGA1551.8189894E-1221.7778135
Produced by FastQC (version 0.11.5)