FastQCFastQC Report
Mon 6 Jun 2016
ERR1378724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596228
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC403.455898E-739.37294844
GTCGGTG250.00210683635.99812337
TCTCGTA458.6941327E-734.99817341
AGAGCGG401.557184E-533.7567335
CCGGCTA551.12668204E-732.72556716
CTCGTAT501.9803101E-631.49835642
CCTTCGT651.3960744E-831.165291
ATCTCGT554.162208E-628.63486940
TCGTATG554.162208E-628.63486943
GCGGTTC507.1511735E-527.0008568
GACCGTG507.155258E-526.9985929
AGACCGT507.155258E-526.9985928
CCGAGAC608.186225E-626.24862925
ATGCCGA651.52276825E-524.22950622
AGCGGTT602.4650487E-422.5007137
CGGCTAA802.9721177E-622.49882717
CCGTGTC602.4664486E-422.49882531
AAGAGCG654.2292365E-420.7733754
GAGCGGT654.2292365E-420.7733756
CGTGTCT654.236426E-420.76814832
Produced by FastQC (version 0.11.5)