FastQCFastQC Report
Mon 6 Jun 2016
ERR1378723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945892
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG651.4015313E-831.1570685
ATGCCGA602.4193832E-729.99834322
GACCGTA654.871945E-727.69224429
CCGAGAC654.871945E-727.69224425
CGTATGC654.8742004E-727.69077944
TCGTATG654.8742004E-727.69077943
CTCGTAT709.3090966E-725.71286642
GAGCGGT801.05232175E-725.3151176
TCTCGTA751.697932E-623.99867641
ATCTCGT802.9738894E-622.49994940
GAGACCG802.9738894E-622.49994927
AGACCGT802.9738894E-622.49994928
GCGGTTC802.9752555E-622.4987588
CGGTTCA855.033058E-621.1753039
ACGGGAT855.033058E-621.17530313
AATGCCG855.033058E-621.17530321
TGCCGAG855.033058E-621.17530323
ACCGTAT908.248882E-619.99995230
CGTATCT908.252653E-619.99889632
CCGTATC908.252653E-619.99889631
Produced by FastQC (version 0.11.5)