FastQCFastQC Report
Mon 6 Jun 2016
ERR1378722.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378722.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782031
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA403.4576442E-739.3730969
CCGTGTA403.4576442E-739.37309631
GAGCGGT458.685438E-735.0050246
AGCGGTT458.69848E-734.998317
AGAGCGG501.978331E-631.504525
CGTATGC501.9803065E-631.50049244
TCGTATG501.9803065E-631.50049243
GCGGTTC501.9812942E-631.4984768
CTCGTAT554.1621934E-628.6368142
TCTCGTA554.1621934E-628.6368141
AGACCGT608.190247E-626.24873228
ATCTCGT651.5227572E-524.23114840
TGCCGAG651.5235115E-524.22959923
AAGAGCG751.6943723E-624.0034454
CATCTCG702.7025742E-522.49891339
GAGACCG702.7025742E-522.49891327
GCGCTCC602.4673383E-422.4989138
CCGAGAC754.6019304E-520.99898525
CGCTCCA654.2379426E-420.7682279
CGTGTAT807.56186E-519.68654832
Produced by FastQC (version 0.11.5)