FastQCFastQC Report
Mon 6 Jun 2016
ERR1378720.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378720.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097326
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTGTATCTCGTATGCC21650.19729779482122906Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT2900.038.79153431
TCGTATG2800.038.56986643
CGTACTG2900.038.01570532
CTCGTAT3000.036.74851242
ACCGTAC3000.036.74851230
CGTATGC2950.036.60868544
TATCTCG3050.036.14607639
TCTCGTA3050.036.14607641
GACCGTA3150.034.9985829
ATCTCGT3300.033.40773840
AGACCGT3350.032.90911528
GAGCGGT3500.032.7933546
AGAGCGG3600.031.8824255
CGGAAGA3400.031.7750031
GAGACCG3500.031.49872427
CCGAGAC3500.031.49872425
GCGGTTC3600.031.2487358
AGCGGTT3750.030.5987637
TCCGAAC300.00514129930.006996
CGCGTCA300.005148177529.99878514
Produced by FastQC (version 0.11.5)