FastQCFastQC Report
Mon 6 Jun 2016
ERR1378715.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378715.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697000
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC551.8189894E-1244.9970632
CGGTTCA700.038.568919
ACCGCTA702.1827873E-1135.3548330
CCGCTAT702.1827873E-1135.3548331
AGACCGC702.1827873E-1135.3548328
TCGTATG702.1827873E-1135.3548343
GAGCGGT754.7293724E-1133.0096866
CGTATGC754.7293724E-1132.99784544
GACCGCT754.7293724E-1132.99784529
TCTCGTA754.7293724E-1132.99784541
ATCTCGT855.456968E-1231.7626340
AGAGCGG801.0004442E-1030.94885
CTCGTAT801.0004442E-1030.93547642
AGCGGGC300.005147529829.99804130
AAGAGCG903.929017E-1027.5100424
GCGGTTC903.947207E-1027.4982038
CCGAGAC903.947207E-1027.49820325
GAGACCG957.403287E-1026.05092827
TGCCGAG957.403287E-1026.05092823
AGCGGTT1001.3442332E-924.7483837
Produced by FastQC (version 0.11.5)