FastQCFastQC Report
Mon 6 Jun 2016
ERR1378714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996474
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC301.140217E-437.4977429
CCGACTA301.140217E-437.4977431
ACCGACT352.8211068E-432.14092330
CGACTAC352.8211068E-432.14092332
CGTATGC406.167737E-428.12330444
AGACCGA450.001226870624.99849728
ACGCCCG500.002265192322.49864612
CCTTCGT1109.9344106E-820.4635811
CTCGTAT550.00393754920.45331442
GCGGTTC550.00393754920.4533148
TATCTCG550.00393754920.45331439
TCTCGAC550.00393754920.45331420
ATGCCGA550.00393754920.45331422
CGCCCGG1151.5958176E-719.5640413
AATGCCG600.00651216618.7488721
GAGACCG600.00651216618.7488727
TCTCGTA600.00651216618.7488741
CCGAGAC600.00651216618.7488725
CCCGCCA1153.433237E-617.6164743
CTTCGTA1153.4497134E-617.6076357
Produced by FastQC (version 0.11.5)