FastQCFastQC Report
Mon 6 Jun 2016
ERR1378713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052940
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGTA803.6379788E-1233.7484732
CTCGTAT801.0004442E-1030.93609642
GAGCGGT901.2732926E-1130.0057666
CCGCTGT901.2732926E-1129.9986431
AGCGGTT901.2732926E-1129.998647
AGACCGC901.2732926E-1129.9986428
GCGGTTC952.3646862E-1128.4197628
TCTCGTA803.3960532E-928.12372441
GACCGCT1004.5474735E-1126.99877429
CGTATGC957.421477E-1026.05144944
AGAGCGG1101.5097612E-1024.550175
ACGCCCG751.6980921E-623.9989112
TCGTATG1052.3683242E-923.5703643
CGGTTCA1152.6557245E-1023.4771989
ACCGCTG1358.367351E-1121.66568430
AATGCCG1257.530616E-1021.59901821
GAGACCG1257.530616E-1021.59901827
TATCTCG1056.1096216E-821.42759939
CGGAAGA1301.2205419E-920.7781561
ATGCCGA1301.2278178E-920.7682922
Produced by FastQC (version 0.11.5)