FastQCFastQC Report
Mon 6 Jun 2016
ERR1378712.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378712.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606792
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG250.002106667335.99881439
AGACCGT250.002106667335.99881428
GTCGTAT250.002106667335.99881433
CGTCGTA250.002106667335.99881432
GACCGTC300.005146194729.99901229
CCGTCGT300.005146194729.99901231
ACCGTCG300.005146194729.99901230
CCGAGAC600.006508274518.74938225
AACGAAG800.001709965616.87444325
CGGCTAA950.0053733214.21005817
CCGGCTA1000.007547266313.49955516
CTTTAGT1352.6420702E-413.3372912
GGTGATC1300.00301941812.11498524
GCACTCC2158.190051E-611.51124916
ACTAGAC3204.7621143E-911.24962919
CCCGGGA1500.00877120910.49965441
CTAGACA4001.382432E-910.12466620
ACCATGC1850.0037024139.7294089
TAGACAG4702.2555469E-109.57415221
GGTTGCA2403.1404517E-49.37469137
Produced by FastQC (version 0.11.5)