FastQCFastQC Report
Mon 6 Jun 2016
ERR1378711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823375
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA451.927583E-839.99913829
CGTACTA451.927583E-839.99913832
ACCGTAC504.8770744E-835.9992230
GCGGTTC651.4028046E-831.1531738
CCGTACT602.418019E-729.99935231
CTCGTAT702.8847353E-828.92794642
TCTCGTA755.6363206E-826.99941841
CGGTTCA801.0532494E-725.3119549
AGCGGTT801.0532494E-725.3119547
CTGTCGG450.001226413424.99946240
CGTATGC751.6969898E-623.99948344
TCGTATG751.6969898E-623.99948343
GAGCGGT903.2814387E-722.5022496
CGCTATA500.00226435322.49951423
CCGAGAC955.5289456E-721.31532925
GAGACCG855.0302897E-621.17601227
ATCTCGT1009.050382E-720.24956340
CATCTCG908.24812E-619.99956939
AGACCGT908.24812E-619.99956928
AGAGCGG1051.4431698E-619.2876425
Produced by FastQC (version 0.11.5)