FastQCFastQC Report
Mon 6 Jun 2016
ERR1378710.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378710.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736029
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGGC250.002107007735.99864226
GACTGCG250.002107007735.99864229
CGTATGC501.9809213E-631.4988144
AGACCGC501.9809213E-631.4988128
CTCGTAT453.4900557E-529.99886742
TCTCGTA453.4900557E-529.99886741
AGAGCGG554.1546864E-628.6430645
CGGAAGA654.8651964E-727.6950261
GAGACCG654.870981E-727.69126327
GAGCGGT651.52082575E-524.2347936
ACCGCAT651.5232288E-524.22985630
GACCGCA651.5232288E-524.22985629
TCGTATG651.5232288E-524.22985643
ATCTCGT602.466969E-422.49915140
AACGGGT500.002264338522.49915112
CCGAGAC802.9733164E-622.49915125
CGCATCA702.7020751E-522.4991532
GCGGTTC754.596266E-521.0020628
AGCGGTT754.596266E-521.0020627
AATGCCG754.6010813E-520.99920821
Produced by FastQC (version 0.11.5)