FastQCFastQC Report
Mon 6 Jun 2016
ERR1378709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723912
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTT250.00210721335.99784531
ACCGCTA751.8189894E-1235.9978430
CCGCTAC751.8189894E-1235.9978431
GACCGCT803.6379788E-1233.7479829
GCGGTTC900.032.4980558
CGCTACA855.456968E-1231.76280632
AGACCGC950.030.7876328
TCTCGTA901.2732926E-1129.99820541
CATACGA300.005147520529.9982057
CGTATGC952.3646862E-1128.41935244
TATCTCG952.3646862E-1128.41935239
ACGCCCG406.1660795E-428.12331612
CTCGTAT1053.6379788E-1227.85547442
CCGAGAC1004.5474735E-1126.99838425
GAGCGGT1105.456968E-1226.5985036
GAGACCG1105.456968E-1226.58931727
ATCTCGT1058.54925E-1125.71274640
TCGTATG1058.54925E-1125.71274643
AGCGGTT1159.094947E-1225.433267
AGAGCGG1101.5097612E-1024.5524645
Produced by FastQC (version 0.11.5)