FastQCFastQC Report
Mon 6 Jun 2016
ERR1378708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981312
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT301.1399672E-437.4990729
GCGGTTC453.4909222E-529.9992528
GAGACCG453.4909222E-529.99925227
GAGCGGT507.156083E-527.0007026
GGAGCGC507.158566E-526.99932741
CGTATGC450.001226604924.99937844
ACCGATA450.001226604924.99937830
TCTCGTA450.001226604924.99937841
AAGAGCG551.3677617E-424.5460934
AGACCGA751.6975919E-623.99940328
GAGCGCT602.467568E-422.49944142
CTCGTAT500.00226470422.4994442
CCGATAG500.00226470422.4994431
GGGCGAC706.983647E-419.28523328
ACAGCCG600.006510769518.74953519
CGATAGT600.006510769518.74953532
AGAGCGG750.001109871918.000475
AGCGGTT750.001110251517.9995527
CGGTTCA800.001710889716.874589
GGCGAAA800.001710889716.8745845
Produced by FastQC (version 0.11.5)