FastQCFastQC Report
Mon 6 Jun 2016
ERR1378705.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378705.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997235
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG458.699826E-734.9992632
GACCGTA453.4908888E-529.99936729
GAGCGGT551.3672907E-424.5473986
TCGTATG551.3682217E-424.54493543
ACCGTAT551.3682217E-424.54493530
CGCCCGG651.5237381E-524.23025513
TTCACGC500.002264679622.49952515
CTCGTAT602.467545E-422.49952342
AGACCGT702.7029719E-522.49952328
GCGGTTC654.2382948E-420.7687918
CCGTATA654.2382948E-420.76879131
CGTATGC706.983578E-419.28530544
TCTCGTA706.983578E-419.28530541
AGCGGTT750.001110241217.9996197
GAGACCG750.001110241217.99961927
CCGAGAC750.001110241217.99961925
TGCCGAG750.001110241217.99961923
ATCTCGT800.001710873416.87464140
CGGTTCA800.001710873416.8746419
CCGGCTA1301.1063195E-515.57659316
Produced by FastQC (version 0.11.5)