FastQCFastQC Report
Mon 6 Jun 2016
ERR1378703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714488
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG601.546141E-1037.49696439
ACCGGGA653.6743586E-1034.61258330
CGTATGC653.6743586E-1034.61258344
CCGAGAC653.6743586E-1034.61258325
TCGTATG801.0004442E-1030.93543
GCGGTTC803.3942342E-928.1227268
GACCGGG803.3942342E-928.12272629
TCTCGTA803.3942342E-928.12272641
AGCGGTT903.947207E-1027.4977767
AAGAGCG901.1834345E-825.0119824
AGAGCGG901.1834345E-825.0119825
CTCGTAT901.1905286E-824.99797842
CGGTTCA901.1905286E-824.9979789
CGGGACA901.1905286E-824.99797832
ATCTCGT1001.3442332E-924.74840
ATGCCGA952.1143933E-823.68229522
CGGAAGA1204.474714E-1022.5139351
GAGCGGT1003.6210622E-822.5107846
AATGCCG1003.6425263E-822.4981821
TGCCGAG1056.104892E-821.4268423
Produced by FastQC (version 0.11.5)