FastQCFastQC Report
Mon 6 Jun 2016
ERR1378702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081119
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT750.041.99740642
CGGGTAA950.033.15738332
CGTATGC1000.031.49805644
TCTCGTA1000.031.49805641
CCGGGTA1100.028.63592131
TCGTATG1150.027.38961243
ACCGGGT1150.027.38834830
GACCGGG1150.027.38834829
CGGTTCA1250.026.9970869
AATGCCG1350.024.99845921
GCGGTTC1350.024.9973038
ATCTCGT1353.6379788E-1223.33405340
ATGCCGA1450.023.27442722
AAGAGCG1600.022.5079844
CGGAAGA1700.021.1879041
GAGCGGT1601.8189894E-1221.1012346
CCGAGAC1501.4551915E-1120.99870325
GAGACCG1501.4551915E-1120.99773427
AGACCGG1501.4551915E-1120.99773428
AGAGCGG1750.020.5787265
Produced by FastQC (version 0.11.5)