FastQCFastQC Report
Mon 6 Jun 2016
ERR1378701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956223
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC800.036.5623744
AGACCGC800.036.5604628
TCTCGTA1050.029.99989741
CTCGTAT1053.6379788E-1227.85704642
GACCGCT1053.6379788E-1227.85558929
GAGACCG1201.6370905E-1124.3736427
GAGCGGT1252.910383E-1123.4084876
ACCGCTG1252.910383E-1123.39869330
GCGGTTC1252.910383E-1123.397478
CGCTGAA1353.6379788E-1223.3320332
CGGAAGA1405.456968E-1222.5128731
GTATGCC1358.367351E-1121.6665945
ATCTCGT1501.4551915E-1120.99992840
AGAGCGG1401.364242E-1020.9004365
TCGTATG1401.364242E-1020.89169143
AGCGGTT1401.364242E-1020.8905987
AAGAGCG1603.8198777E-1119.6946414
CCGCTGA1603.8198777E-1119.68640131
CCGAAAA600.006513777718.74797213
CCGAGAC1454.773028E-918.6196525
Produced by FastQC (version 0.11.5)