FastQCFastQC Report
Mon 6 Jun 2016
ERR1378698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269041
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT750.038.99706331
ACCGGTA900.032.49755530
CGTATGC950.030.78836644
TCTCGTA950.030.78836641
CGGTATT1001.8189894E-1229.24779732
CTCGTAT1053.6379788E-1227.85614242
GACCGGT1004.5474735E-1126.99796729
TCGTATG1105.456968E-1226.58890743
ATCTCGT1159.094947E-1225.4338740
GAGACCG1159.094947E-1225.43186627
GAGCGGT1252.910383E-1123.4111546
AGCGGTT1252.910383E-1123.3973167
AAGAGCG1459.094947E-1221.7344914
AGACCGG1358.367351E-1121.66503728
CATCTCG1452.1827873E-1020.1716939
CGGAAGA1503.4197E-1019.5154481
GCGGTTC1555.347829E-1018.8688058
CGGTTCA1709.276846E-1118.5272879
CCGAGAC1507.2777766E-917.99793425
AGAGCGG1651.2223609E-917.7357225
Produced by FastQC (version 0.11.5)