FastQCFastQC Report
Mon 6 Jun 2016
ERR1378697.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378697.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044259
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT750.042.031
TCGTATG900.034.9983243
CGTATGC950.033.15789444
ACCGCTA950.033.15630730
TCTCGTA950.033.15630741
CGCTATT1100.032.72727232
GCGGTTC1050.032.1397828
CTCGTAT1000.031.49849142
GAGCGGT1100.030.6921046
AGACCGC1100.028.6349928
CGGTTCA1200.028.1223059
AGCGGTT1250.026.9974167
TGCCGAG1250.026.99741623
ATCTCGT1251.8189894E-1225.19879340
AGAGCGG1350.025.0083835
AATGCCG1350.024.99760821
CCGAGAC1405.456968E-1222.49784725
AAGAGCG1551.8189894E-1221.7814944
ATGCCGA1551.8189894E-1221.77210822
GAGACCG1459.094947E-1221.72205727
Produced by FastQC (version 0.11.5)