FastQCFastQC Report
Mon 6 Jun 2016
ERR1378688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167113
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACTTATCTCGTATGCC11990.10273212619514992Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2050.036.2173444
TATCTCG2000.034.87290639
TCTCGTA2000.034.87290641
TCGTATG2150.033.48636643
ATCTCGT2100.033.21229640
CTCGTAT2100.033.21229642
ATGCCGA2250.032.99660522
CCGTGAC2150.032.43991531
AGAGCGG2450.032.1574635
GCGGTTC2450.032.1395538
CGGTTCA2450.032.1395539
GAGACCG2350.031.59249527
GAGCGGT2500.031.514316
AGCGGTT2550.030.8791777
CGTGACT2350.030.6364632
AAGAGCG2600.030.3022234
AATGCCG2500.029.69694721
ACCGTGA2350.029.677830
AGACCGT2450.029.38473528
GACCGTG2500.028.79703929
Produced by FastQC (version 0.11.5)