FastQCFastQC Report
Mon 6 Jun 2016
ERR1378687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308433
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA1500.040.49965731
TCTCGTA1650.038.18149641
ATCTCGT1850.035.2699740
AGACCGC1700.034.41015628
CGTATGC1850.034.0550744
CGCTTAT1850.034.05376432
CTCGTAT1900.033.1576142
ACCGCTT1850.032.83630430
GACCGCT1850.031.62014629
AGCGGTT2150.031.3902827
TCGTATG2100.030.0008943
ATGCCGA2400.029.05892422
GCGGTTC2350.028.718778
AGAGCGG2450.027.5602655
AAGAGCG2550.027.3631674
GAGACCG2150.027.20699127
CCGAGAC2300.026.41079925
GAGCGGT2600.025.9702536
AATGCCG2750.025.36051621
CGGAAGA2700.025.013171
Produced by FastQC (version 0.11.5)