FastQCFastQC Report
Mon 6 Jun 2016
ERR1378685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308874
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1200.035.6237941
CTCGTAT1150.035.21619442
GCGGTTC1300.032.8822448
CCGCTGA1350.029.99898331
GACCGCT1350.029.99783529
AGACCGC1350.029.99783528
AGAGCGG1450.029.4907685
GAGCGGT1400.028.9364346
TCGTATG1400.028.9275943
CGGTTCA1400.028.9264839
AGCGGTT1500.028.4979447
TATCTCG1450.027.93008639
ACCGCTG1450.027.92901830
GAGACCG1450.027.92901827
CGTATGC1500.026.99908344
CGCTGAT1500.026.99908332
TGCCGAG1650.024.54368223
CCGAGAC1750.024.4268125
ATCTCGT1700.023.82272140
AAGAGCG1850.023.1143884
Produced by FastQC (version 0.11.5)