FastQCFastQC Report
Mon 6 Jun 2016
ERR1378684.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378684.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053234
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGATTATCTCGTATGCC15040.14279827654633254Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC2250.039.9963729
ACCGTCG2350.038.294430
CGTATGC2350.037.3388144
TCGATTA2350.037.3388134
AGACCGT2450.036.7313628
GTCGATT2400.036.56091733
CGATTAT2400.036.56091735
TCGTATG2400.036.56091743
ATCTCGT2500.035.99844740
CCGTCGA2450.035.81477731
CGTCGAT2500.035.09848432
CTCGTAT2550.034.4102842
GAGACCG2650.033.95918327
TCTCGTA2650.033.11177441
TATCTCG2700.032.49859639
GCGGTTC2800.032.1384128
AGAGCGG2900.031.050835
AGCGGTT2900.031.0301937
GAGCGGT2950.030.5245486
CGGTTCA3100.029.0282469
Produced by FastQC (version 0.11.5)